ARTICLE

Gonadal Development, Spawning and Plasma Sex Steroid Levels of the Indoor Cultured Grunt, Hapalogenys nitens

Hee Woong Kang1, Jae-Kwon Cho, Maeng-Hyun Son, Jong Youn Park†, Chang Gi Hong, Jae Seung Chung2, Ee-Yung Chung3
Author Information & Copylight
Corresponding Author : Jong Youn Park, Southwest Sea Fisheries Research Institute, Yeosu 556-823, Korea. Tel. : +82-61-690-8975, Fax : +82-61-685-9073, E-mail: 5556660@naver.com

Copyright © 2014 © Copyright an Official Journal of the Korean Society of Developmental Biology. All Rights Reserve. This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Received: Jan 28, 2015 ; Revised: Feb 10, 2015 ; Accepted: Feb 25, 2015

ABSTRACT

The gonadosomatic index (GSI), gonadal development and changes in hormones in plasma level of the indoor cultured grunt (Hapalogenys nitens) were investigated by histological study from August 2011 to October 2012. The GSI showed similar trends with gonad developmental stages during the culture periods. Changes in plasma level of estradiol-17β of female H. nitens reached the highest value before the spawning period, and seasonal changes in plasma level of estradiol-17β were similar in trends of oocyte developments and GSI changes. Testosterone levels of male H. nitens reached the highest value before and after the spent stage. Ovarian developmental stages of H. nitens could be classified into early growing stage, late growing stage, mature stage, ripe and spawning stage, recovery and resting stage. The testicular developmental stages could be divided into growing stage, mature stage, ripe and spent stage, and recovery and resting stage.


INTRODUCTION

The grunt, Hapalogenys nitens, which belongs to the Perciformes (Haemulidae), is found in all coastal waters of Korea, south Japan, and eastern China Sea (NFRDI, 2004). The body type of H. nitens is characterized with high body height, two clear and wide dark brown colored lines on the side of body; a similar kind, crescent sweetlips, Plectorhinchus cinctus, is distinguished by spots on the back and caudal fin (NFRDI, 2004). H. nitens grows fast and represents strong resisting power against diseases, making it as a good potential fishery species for development of novel aquaculture species in southern coastal area of Korea inclu-ding Tong-young area. In recent, the National Fisheries Research and Development Institute is currently conducting preliminary investigations for species conservation in order to study Korean indigenous species conservation as well as seed production.

Previously, there have been many studies on H. nitens,: on aspects of reproduction, including vitellogenesis (Cuiqin et al., 2006), natural spawning and egg development (Kang et al., 2004a), morphological development (Xie et al., 2004), development of seed production technology (Zhang et al., 2001a; Hong and Zhang, 2002, 2003), on aspects of ecology, including distribution and morphology (Masuda et al., 1984; Lee et al., 1997; Lim and Choi, 2009), effects of water temperature and salinity on hatching and larval survival (Lin et al., 1998), effect of salinity on activity and larval feeding rate (Zheng et al., 2004), effects of feeds on growth and survival of juveniles (Zhang et al., 2003), effects of low salinity and cold water temperature on growth and survival rates of eggs and offspring (Kang et al., 2009), on aspects of aquaculture, including growth performance in cage aquaculture (Li et al., 2007), early nutritional com-positions (Zhang et al., 2001b; Limin et al., 2006), and on aspects of genetics, including karyotypes (Ziniu et al., 1994; Chen et al., 2005), genetic diversity (Liang et al., 2003), microsatellite separation for genetic analyses (An et al., 2014) of this species.

Although, there are several studies, there are still gaps in our knowledge on reproduction and aquaculture. Little information is available on the reproductive cycle, the use of sexual reproductive hormones associated with sexual maturation and seedling production of H. nitens. Regarding the development of aquaculture technology, recently, sexual reproductive hormone control have been studied for artificial spawning and rapid growth. Thus, the use of sexual reproductive hormone control will contribute to develop aquaculture technology of this species. Hence, it is expected that information for technology development of massive seedling production of H. nitens would be clarified.

Therefore, the purpose of the present study is to describe basic information of the gonadosomatic index (GSI), repro-ductive cycle with gonadal development, and plasma sex steroid studies such as use of changes in estradiol-17β and testosterone in plasma in female and male H. nitens for aquaculture.

MATERIALS AND METHODS

1. Changes in hormonal levels in plasma of H. nitens

A total of 30 H. nitens (total length: 38.2 ± 0.2 cm; body weight: 1,577.7 ± 31.7 g), which were maintained and cultured from August 2011 to July 2012 at the Yellow Sea Fisheries Research Institute of NFRDI, were used for the study. The culture conditions were as follow: fishes were maintained in 10 tons-concrete circular tank; natural sea water was changed 6 times per day utilizing a high pre-ssure sand filter while assorted feed for flatfish, Paralichthys olivaceus was provided twice a day. In the winter season, water temperature was heated, maintained at 11.5~26.5°C year round. And salinity was maintained with the salinity concentration of 30.1 and 33.9 psu.

When it comes to the culture management, seven stages were subdivided: spawning period (29 August 2011), after spawning (1 October 2011), before wintering (26 November 2011), wintering period (19 February 2012), after wintering (21 April 2012), culture period (27 May 2012), and before spawning (27 July 2012).

To monitor changes in hormonal levels of plasma, each one of fishes in the tank was retrieved and then plasma sample was taken from tail vein utilizing a heparin treated syringe; upon the sample was taken, the fish was immediately transferred to the tank and recovered. The plasma sample was separated by spinning at 12,000 rpm for 5 minutes using a centrifuge which was maintained at 4°C. Separated plasma sample was stored at –75°C until further analyses. Plasma steroids were extracted using the method as described previously (Aida et al., 1984); extracted steroids were then stored at –70°C for the radioimmuno-assay (RIA assay). For the quantification of steroid hormones, the RIA method, previously described in Aida et al. (1984) and Lou et al. (1984), was utilized. The estradiol-17β and testosterone were quantified in female and male fishes, respectively. Antibodies used for the quantification were obtained from Teikoku Zoki Pharm. Co.; the cross-reaction rates of these antibodies were found to be 3.2, 1.77, and 0.29% for estrone, estradiol, and testosterone, respectively.

2. Investigation of the gonadosomatic index (GSI) and gonad development of H. nitens

The GSI was calculated by (gonad weight/body weight) × 100. Histological observations for both female and male gonadal developments were made; three fishes were retrieved per each culture stages, for a total of six times: before spawning (11 August 2011), after spawning (18 November 2011), wintering period (18 March 2012), after wintering (21 April 2012), culture period (27 June 2012), and shortly after spawning (16 October 2012).

For light microscopic examination of histologic pre-parations, female ovarian tissues and male testicular tissues were removed from the gonads and preserved in Bouin fixative for 24 h, and then washed running tap water for 24 h. The tissues were subjected to standard histological procedures (dehydrated in alcohol and embedded in paraffin) and sectioned at 5~8 μm using a rotary microtome. Sections were then mounted on glass slides, stained with either Hansen’s hematoxylin-0.5% eosin, and inspected under a light microscope. After histologic preparations produced by the methods mentioned earlier, prepared tissue sections were then analyzed and monitored for shapes and sizes of germ cells utilizing an optical microscope (Axioskop 2 plus; Carl Zeiss, Jena, Germany) interfaced with the image analysis system (AxioVision Rel., ver. 4.6).

To monitor ovarian and testicular developmental phases, shapes and structures of germ cells were histologically analyzed. All histological terms used for designating cellular structures were adopted as reported in the study of Grier et al. (2009) in the “Reproductive Biology and Phylogeny” published by Jamieson in 2009; recently, all terms are internationally certified and widely utilized in other studies.

All results herein were expressed as mean±S.E and statistical differences of means between groups were deter-mined using t-test and one way-ANOVA test (SPSS package, ver. 9.0) at P value of 0.05.

RESULTS

1. Changes in GSI

Changes in GSI from August 2011 to October 2012 were depicted in the Fig. 1. The average value of GSI in females was the highest in August 2011 (GSI, 5.2) yet it was lower than after November (GSI, 2.3). On the other hand, in male H. nitens, the GSI reached the highest value in November 2011 (GSI, 1.7) and then gradually decreased; it reached the lowest value from April to June 2012 (GSI, 0.2) but increased up to October 2012 (GSI, 1.2).

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Fig. 1. Monthly changes in the gonadosomatic index (GSI) of indoor cultured Hapalogenys nitens.
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2. Changes in plasma hormones

Quantitative changes in plasma hormones of cultured H. nitens were shown in the Fig. 2 and Fig. 3. The level of estradiol-17β in female H. nitens was 0.055 ± 0.020 ng/mL in August 2011 (the spawning period), while it was largely decreased in October 2011 (0.013 ± 0.004 ng/mL), as the period of after spawning period. And then, their values are continuously decreased, and reached the minimum value (0.003 ± 0.003 ng/mL) in February 2012 (the wintering period). Thereafter, it was gradually increased up to the culture period and reached the maximum value (0.076 ± 0.014 ng/mL) in July 2012 (before spawning period). In the present study, no statistical difference can be found in levels of hormones in August 2011 (the spawning period) and July 2012 (before spawning); however, there was a significant difference between October 2011 (after spawning period) and May 2012 (the culture period; P<0.05).

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Fig. 2. Monthly changes in estradiol-17β in female Hapalogenys nitens from August 2011 to July 2012.
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Fig. 3. Monthly changes in testosterone contents in male Hapalogenys nitens from August 2011 to July 2012.
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In contrast, in male H. nitens, the level of testosterone was the maximum in August 2011 (1.688 ± 0.511 ng/mL) during the spent period, and then it was drastically decreased in October 2011 (0.058 ± 0.009 ng/mL), after the spent period, which was the lowest level throughout the study. After this, in November 2011, the level of testos-terone was increased (before wintering; 1.556 ± 0.518 ng/mL), and then decreased again in the wintering period (February 2012; 0.610 ± 0.183 ng/mL).

In the culture period (May 2012), the testosterone was shown to be low values as 0.037 ± 0.037 ng/mL, while it was increased in July 2012 (before spent period, 0.407 ± 0.056 ng/mL). In the results herein, there was no differ-rence between the spent period (August 2011) and before the wintering period (November 2011). However, it was statistically different between October 2011 (after the spent period) and February 2012 (the wintering period; P<0.05).

3. Gonad developmental phases

Based on the morphological features and sizes of germ cells and tissue cells around them by histological characteristics, ovarian developmental stages in ovaries in female H. nitens can be classified into five successive stages: early growing, late growing, mature, ripe and spawning, and recovery and resting stages. However, testicular developmental stages in testes in male H. nitens can be divided into four stages: growing, mature, ripe and spent, and recovery and resting stages.

4. Ovary
1) Early growing stage

During the period of wintering in late-March (water temperature, 11°C), female individuals were heating cultured. At this time, ovarian development was relatively weak in the early growing stage: it was specifically characterized with chromatin nucleolus oocytes and perinucleolar oocytes of the primary growth stage in the ovarian lobules. These oocytes were 20.4~67.8 μm in diameter, and also a few cortical alveolar oocytes of the primary growth stage were found whose size were 87.8~155.9 μm in diameter (Fig. 4A).

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Fig. 4. Photomicrographs of ovarian developmental stages in female Hapalogenys nitens. A, Sections of ovarian lobules in the early growing stage. Note morphological characteristics of chromatin nucleolus, perinucleolar and cortical alveolar oocytes in the ovarian lobules; B, Sections of ovarian lobules in the late growing stage. Notes the perinucleolar, cortical alveolar, and oil droplet oocytes and early yolked oocytes and late yolked oocytes including oil droplets primary yolk granules in the lobules; C, Sections of ovarian lobules in the late growing stage. Notes oocytes including primary yolk granules, secondary yolk granules, tertiary yolk granules in lobules in the yolk stage; D. Sections of ovarian lobules in the mature stage. Note ecentric germinal vesicle, oil globule, a zona pellucida and follicle cells in mature oocytes in the lobules. E. Sections of ovarian lobules in the ripe and spawning stage. Note undischarged oocytes, degenerated oocytes showing residual trace of postovulatory follicles and atretic follicles in ovarian lobules; F, Sections of ovarian lobules in the recovery and resting stage. Note a number of chromatin nucleolus and peri-nucleolar oocytes degenerated in the lobules. Abbreviations: AF, atretic follicle; FC, follicle cells; gv, germinal vesicle; N, Nucleus; OD, oil droplets; og, oil globule; OL, ovarian lumen; OMegv, oocyte maturation ecentric germinal vesicle; PGca, cortical alveolar oocyte; PGod, oil droplets oocyte; PGpn, perinucleolar oocyte; POF, postovulatory follicle; PYG, primary yolk globules; SYG, secondary yolk globules; TYG, tertiary yolk globules; ZP, zona pellucida. Scale bar 200 μm.
Download Origianl Figure
2) Late growing stage

After wintering, natural sea water was used for culturing in late-April (water temperature, 13.0°C). At this stage, ovarian development in ovarian lobules was remarkably developed. In particular, chromatin nucleolus oocytes (20.4~22.3 μm in diameter) and perinucleolar oocytes (60.5~70.8 μm in diameter) of the primary growth stage as well as further developed cortical alveolar oocytes (or yolk vesicle oocytes) in the late growing stage; in this stage, the numbers of oocytes were relatively high yet their diameters were small, stained with dark blue color by hematoxylin staining. In contrast, germ cells were found in the early growing stage were shown to be more developed early secondary growth stage oocytes; in these oocytes, diameters of early yolked oocytes of 115.2~165.5 μm contained oil droplets (Fig. 4B). However, in late-June (water temperature 22.4°C), late yolked oocytes (210.3~252.7 μm in diameter) containing oil droplets, second and tertiary yolk globules of the late secondary growth stage were found in ovarian lobules; the zona pellucida is located in the outer membrane of the oocyte, and the follicle cells are located on the outer layer of the zona pellucida (Fig. 4C).

3) Mature stage

Female H. nitens, which found between late-July (water temperature 26.5°C) and mid-August (water temperature 25.5°C), are before the spawning period and a number of full-grown oocytes (369~459 μm in diameter) appeared in the ovarian lobules, as well as some of early mature oocytes; in these full-grown oocytes, secondary and tertiary yolk granules (or globules) appeared in the ooplasm homo-genized. In this stage, germinal vesicles of oocytes were shrunk, and moved to the animal pole, and then they disappeared. Around yolk globules, there are multiple oil globules, and a number of small oil droplets were found in all around ooplasm. Ooplasms of mature eggs were acidophil as stained with eosin (Fig. 4D).

4) Ripe and spawning stage

In late-August (water temperature, 25.5°C), fully riped eggs (or full-grown oocytes) began to start ovulation from ovarian lobules. In mid-October (water temperature, 25.5°C), postovulatory follicles and atretic follicles were observed in ovarian lobules with residual traces. Ooplasms of chromatin nucleolus and perinucleolar oocytes (approximately 42.8~70.9 μm in diameter), which were found in the primary growth stage, became non-basophilic, and decolored (Fig. 4E).

5) Recovery and resting stage

After spawning, in mid-November (water temperature 11.4°C), ovarian lobules were degenerated/shrunk up to two months from the spawning period; chromatin nucleolus and perinucleolar oocytes (23.5~67.8 μm in diameter) of the primary growth stage were found in ovarian lobules with decolored basophilic cytoplasm (Fig. 4F).

5. Testis
1) Growing stage

In testicular lobules of male H. nitens found between late-June (water temperature 22.4°C) and mid-July (water temperature 25.4°C), spermatogenesis began to start; spermatocytes, spermatids and sperms during spermiogenesis were found in testicular lobules (Fig. 5A).

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Fig. 5. Photomicrographs of testicular developmental stages in male Hapalogenys nitens. A, Sections of testicular lobules in the growing stage. Note a numbers of spermatogonia, spermatocytes, spermatids, and small number of spermatozoa in the testicular lobules. B. Sections of the lobules in the mature stage. Note a numbers of spermatozoa and spermatids in the lobules. C, D, Sections of testicular lobules in the ripe and spent stage. Note undischarged spermatozoa, residual spermatids in the lobules; E, F, Sections of testicular lobules in the recovery and resting stages. Note degenerated spermatozoa and spermatids in the degenerated testicular lobules, and newly formed spermatogonia in the lobules. Abbreviations: DST, degenerated spermatid; DSZ, degenerated spermatozoon; DTL, degenerated testicular lobule; LUTL: the lumen of the testicular lobule; RSZ, residual spermatozoon; ST, spermatid; SZ, spermatozoon; Scale bar 100 μm.
Download Origianl Figure
2) Mature stage

Most male H. nitens found between late-July (water temperature 26.5°C) and mid-August (water temperature 25.5°C) contained many testicular lobules, which were filled with a number of spermatozoa and spermatids as well as small number of spermatocytes (Fig. 5B).

3) Ripe and spent stage

Between late-August (water temperature 25.5°C) and mid-October (water temperature 20.4°C), male H. nitens contained many testicular lobules, which were fully filled with undischarged sperms in the ripe and spent stage (Fig. 5C). After mid-November (water temperature 11.4°C), most fishes were after spent stage. At this time, testicular lobules contained remaining undischarged sperms which were in the center of lobules; a number of sperms were degenerated while edges of lumen of testicular lobules were shown to be empty as most sperms were discharged (Fig. 5D).

4) Recovery and resting stage

While wintering, between late-March (water temperature 11.5°C) and early-April (water temperature 13.0°C), most male H. nitens had degenerated or shrunken testicular lobules in the testes. Therefore, it was difficult to find their original shapes as well as germ cells (Fig. 5E). Even after wintering, from the resting stage to late April (water temperature 13.1°C), testicular lobules were alike as shown previously. In this, a few spermatocytes were found and monitored the early stage of spermatogenesis (Fig. 5F).

DISCUSSION

H. nitens is one of the subtropical species and has been gaining much attention for fish farming as they are frequently harvested in both Southern and Western coast of Korea possibly due to elevated water temperature from global warming (Lim & Choi, 2009). Given its significance as a novel aquaculture species, it is timely and important to elucidate biological factors as well as accurate gonad developmental stages of H. nitens in indoor culture for the species conservation as well as development of aquaculture technology. To date, regarding our knowledges of this species, little information is available with regards to artificial indoor culture and breeding ecology of H. nitens hence studies about their maturation and spawning are significant.

Most teleostean fishes are matured, and spawn in certain periods; there is periodicity for developmental changes of intra-structures of reproductive organs, mainly around their spawning stage. These periodical changes are known to be modulated by various hormones secreted from endocrino-logical system which can be stimulated via multiple environmental factors such as water temperature as well as photoperiod (Stressmann et al., 1996; Kang et al., 2004b; Kang et al., 2008; Kang et al., 2012).

Generally, the GSI is calculated in order to indirectly estimate the spawning period. Changes in the GSI of cultured female H. nitens in the landbased fish culture tank was found to be the highest in August (GSI, 5.2) while male H. nitens had the highest value in November (GSI, 1.7) followed by gradual reduction which was similar results demonstrated using wild H. nitens in which fishes were induced for natural spawning in between August and September (Kang et al., 2004a).

Trends of changes in plasma estradiol-17β of female H. nitens were somewhat in agreement with development of oocytes and GSI changes. It has been known that estradiol-17β modulates formation of vitellogenin (Nagahama, 1987). In the study, we found that this hormone was gradually increased from July when vitellogenin made for H. nitens followed by reduction in early-October (after spawning stage) which was also demonstrated in H. otakii (Lee et al., 2000). Fish testes secret steroid hormones in response to stimulation of GTH produced and secreted by the pituitary gland. Of these steroid hormones, it has been reported that testosterone is produced from steroid hormone producing of Leydig cells of interstitial tissue and involved in spermatogenesis as well as secondary sexual characteristics development (Nagahama et al., 1998). The level of testosterone in male H. nitens was shown to be highest before and after the spent stage which is in agreement with spotlined sardine, Sardinops melanostictus (Matsuyama et al., 1991), and H. otakii (Lee et al., 2000).

In our study, the ovarian development of H. nitens was getting into the ripe-spawning stage in between mid-August and September as the maximal GSI value was found in this period. In the recovery and resting period, after mid-November, ovarian lobules are degenerated and shrunk so that basophilic chromatin nucleolus and peri-nucleolar oocytes were observed. In the recovery and resting period, as demonstrated in other studies [e.g., T. obscurus (Kang et al., 2008)], RNA is extruded into cytoplasm of chromatin nucleolus and perinucleolar oocytes thereby representing dark blue color in an optical micro-scope upon staining with hematoxylin. In the testis of H. nitens, a number of testicular lobules are present; in their mature stage, in mid-August, most male H. nitens have testicular lobules filled with sperms. From the wintering period to late-April, testicular lobules were degenerated and shrunk as the recovery and resting stages are main-tained for a while. In mid-October, right after spent sperms, all testicular lobules were filled with sperms.

Taken altogether, in the present study, the authors studied the artificial indoor cultures and breeding of H. nitens via investigating gonadal development and changes in plasma sex hormones; these results herein are expected to be utilized as an important preliminary data for artificial breeding of H. nitens.

ACKNOWLEDGEMENTS

This research was supported by the Preliminary Research Grant (RP-2015-AQ-017) for Fisheries of National Fisheries Research & Development Institute.

REFERENCES

1.

Aida K, Kato T, Awaji M. Effects of cartration on the smoltification of precocious male masu salmon, Oncorhynchus masou. Bull Jpa Soc Fish. 1984; 50:565-671.

2.

An HS, Kang HW, Han HS, Park JY, Myeong JI, An CM. Isolation and characterization of 26 novel poly-nucleotide microsatellites from short barbeled grunter (Hapalogenys nitens) for genetic analysis. Conservation Genet Resour. 2014; 6:669-672.

3.

Chen X, Wang S, Wang J, Wang D. Karyotypes of cultured Hapalogenys nitens from Xiamen stock. J Xiamen Univ. 2005; 44:200-202.

4.

Cuiqin W, Dongxing Y, Baomin L. Rapid deter-mination of vitellogenin in fish plasma by anion ex-change high performance liquid chromatography using postcolumn fluorescence derivatization with o-phthalal-dehyde. Analytical Sci. 2006; 22:1593-1596.

5.

Grier HJ, Uribe Araanzabal MC, Pattino RR. In: Jamieson BGM, editor. The ovary, folliculogenesis, and oogenesis in teleosts. Reproductive Biology and Physiology of Fishes (Agnathans and Bony Fishes). 2009; 8A St. Lucia, Queensland, Australia: The University of Queensland. p. 25-84.

6.

Hong WS, Zhang QY. Artificial propagation and breeding of marine fish in China. Chin J Oceanol Limnol. 2002; 20:41-51.

7.

Hong WS, Zhang QY. Review of captive bred species and fry production of marine fish in China. Aquacult. 2003; 227:305-318.

8.

Kang HW, Chung EY, Kang DY, Park YJ, Jo KC, Kim GH. Gonadal maturation and spawning of river puffer Takifugu obscurus indoor cultured in low salinity. J Aquacult. 2008; 21:331-338.

9.

Kang HW, Chung EY, Kim JH. Sexual maturation and spawning characteristics in Greenling, Hexagrammos otakii of the west coast in Korea. J Aquacult. 2004b; 17:30-38.

10.

Kang HW, Jun JC, Kang DY, Jo KC, Choi KH, Kim GH. Influence of low salinity and cold water tem-perature on the hatching, survival and growth of the offspring of grunt, Hapalogenys nitens. Korean J Ichthyol. 2009; 21:158-166.

11.

Kang HW, Kim JH, Ryee KH, Kim JS. Natural spawning and characteristics of egg development of the indoor cultured Grunt, Hapalogenys nitens. J Aquacult. 2004a; 17:180-186.

12.

Kang HW, Lim HG, Kang DY, Han HS, Do YH, Park JS. Maturation and spawning of the female tongue sole, Cynoglossus semilaevis in the west coast in Korea. Dev Reprod. 2012; 2:87-93.

13.

Lee JK, Lim HG, Han CH, Jeung JH, Kim DJ, Aida K. Changes of gonadosomatic index and sex steroid hormone of serum in cultured greenling (Hexagrammos otakii). J Korean Fish Soc. 2000; 33:302-306.

14.

Lee TW, Moon HT, Choi SS. Change in species composition of fish in Chonsu Bay(II) surf zone fish. Korean J Ichthyol. 1997; 9:79-90.

15.

Li J, Zhang JQ, Ou YJ, Zhang JS, Liu Z, Liao R. Study on the growth performance of skewband grunt Hapalogenys nitens in sea gulf net cage. South China Fish Sci. 2007; 3:1-6.

16.

Liang J, Wang J, Su Y, Cai Y, Wang D. Isoenzyme analysis of genetic diversity in cultured Hapalogenys nitens. J Oceanogr Taiwan Strait. 2003; 22:19-23.

17.

Lim HC, Choi Y. Fish fauna of the coastal waters off taean in the west sea of Korea. Korean J Ichthyol. 2009; 12:215-222.

18.

Limin L, Feng X, Jing H. Amino acids composition difference and nutritive evaluation of the muscle of five species of marine fish, Pseudosciaena crocea (large yellow croaker), Lateolabrax japonicas (common sea perch), Pagrosomus major (red sebream), Seriola dumerili (Dumeril' amberjack) and Hapalogenys nitens (black grunt) from xiamen bay of China. Aquacult nut. 2006; 12:53-59.

19.

Lin W, Cai F, Chen W, Chen K, Lai X, Huang M. Effects of different temperature and salinity on hatching larval survival rate Hapalogenys nitens. J Oceanogr Taiwan Strait. 1998; 17:305-308.

20.

Lou SW, Aida K, Hanyu I, Sakai K, Nomura M, Tanaka M, Tazaki S. Endocrine profiles in the females of a twice-annually spawning strain of rainbow trout, Salmo gairdneri. Gen Comp Endocriol. 1984; 64:212-219.

21.

Masuda H, Amaoka K, Araga C, Uyeno T, Yoshino T. The fishes of the Japanese Archipelago. 1984; 1 Tokyo, Japan: Tokai Univ Press. p. 437.

22.

Matsuyama M, Adachi C, Nagahama Y, Kitajima C, Matsuura S. Testicular development and serum levels of gonadal steroids during the annual reproductive cycle captive Japanese sardine. Japan J Ichthyol. 1991; 37:381-390.

23.

Nagahama Y. Gonadotropin action on gametogenesis and steroidgenesis in teleost gonads. ZoolSci. 1987; 4:209-222.

24.

Nagahama Y, Kobayashi T, Chang XT, Nagahama Y. Gonadal sex differentiation in teleost fish. J Exp Zool. 1998; 281:362-373.

25.

NFRDI Commercial fishes of the costal & offshore waters in Korea. Hangul. 2004; p. 333

26.

Stressmann CA, Takashima F, Toda K. Sex differrentiation and hormonal feminization in pejerrey Odontesthes bonariensis. Aquacult. 1996; 139:31-45.

27.

Xie Y, Zhang Y, Hu J, Sun B, Zhong Y, Lin L. Morphological studies of early development of Hapalogenys nitens. J Fish Sci China. 2004; 11:89-94.

28.

Zhang Q, Hong W, Chen P. Status and prospects of artificial propagation and breeding technique of marine fish in Fujian. J Oceanolgr Taiwan Strait. 2001a; 20:266-273.

29.

Zhang W, Su Y, Wang J, Quan C, Ding S. Biochemical composition of five common reared fishes. Mar Sci Bull. 2001b; 20:26-31.

30.

Zhang Y, Hu J, Xie Y, Zhong Y, Huang C. Effects of diets on growth and survival rates of artificially-produced juveniles of Hapalogenys nitens. Mar Sci. 2003; 27:30-33.

31.

Zheng L, Fang Q, Wang H, Zhang J. Effect of salinity on activity and larval feeding rate Hapalogenys nitens Richardson. Mar Sci. 2004; 28:5-7.

32.

Ziniu Y, Xiaoyu K, Wenwu X, Zongyang X. The karyotypes of Hapalogenys nitens (Richardson) and H. mucronatus (Eydoux et Souleyet). J Ocean Univ Qingdao Haiyang Daxue Xuebao. 1994; 24:175-180.