Table 1.
Clinically important down-regulated miRNAs in OS

miRNAs Analysis of samples with respect to OS tissue and cell lines Putative function Chromosome number Targets of miRNA Techniques used for validation References
miR-199a-3p, miR-127-3p OS cell line vs osteoblast cells Inhibits cell growth, migration, regulate cell cycle 19, 14 respectively mTOR, stat3 Microarray, qRT-PCR Duan et al. (2011)
miR-34 family (34a, 34b, and 34c) OS cell line vs patients samples P53 dependent manner cell cycle arrest and apoptosis 1, 11 respectively CDK6, E2F3, CyclinE2, Bcl-2 qRT-PCR, 3UTR luciferase reporter assay, FACS analysis, DNA methylation assay He et al. (2009); Wu et al.(2013)
miR-382, miR- 134, miR-369- 3p and miR-544 OS vs normal bone, OS cell line vs osteoblast 14q32 miRNAs decreased cMYC levels and induced apoptosis 14 cMYC Microarray, qRT-PCR, Western blot Thayanithy et al. (2012)
miR-143 OS patients tissue sample,MG63 and U2OS cell lines vs normal osteoblast and HEK293 cell line miR-143 may exert its proapoptoticfunction via inhibiting Bcl-2 expression. 5 Bcl2 3 UTR luciferase reporter assay, qRT-PCR, Western Blot and Tumorigenicity assay in nude mice Zhang et al. (2010)
miR-145 Human OS tissues vs normal boneTissues, MG63 vs ECV304 cell lines miR-145 down regulate VEGF attranslational level by partially binding to VEGF 3 UTR. 4 BCL2L2, MCL-1,IGF1R and ME, p-Akt and FAK qRT-PCR, FACS analysis, Cell Proliferation Assay, Boydenchamber assay, Western blotting and Hierarchical cluster analysis Zhao et al. (2013)
miR-24 Human OS vs non-cancerousadjacent tissues, MG63, 143Band hFOB1.19 Overexpression of miR-24 downregulatedLPAATb expression in OScells. 9 LPAATb Immunofluorescence andImmunohist. staining,Computational analysis byprogram:TargetScan5.1, qRTPCRanalysis, Fluorescentreporter assay, Cell viabilityassay, FACS Song et al.(2013)
miR-16 HOS, KHOS, U2OS, and MG-63 vs HOB cell line andEighteen OS patient samplesvs normal tissues Overexpression of miR-16suppresses OS cell proliferation andtumor growth by inhibition of Raf1 MEK1/2 ERK1/2 pathway. 13 IGF1R Cell proliferation assay and cellcycle analysis, Western blotting,Tumor growth assay, luciferasereporter assay, Chen et al.(2013)
miR-199b-5p Eight FFPE Patients vstissue samples, ES97, HS3.T,SJSA-1, andMG63 vs normal cell line Inhibition of Notch and HES1signaling by miR-199b-5p as apotential therapeutic strategy toprevent metastasis in human OS. 9 HES1 andNotchsignaling Microarray analysis and qRTPCR Won et al.(2013)
miR-124 MG-63, U2OS, SOSP-9607,and SAOS-2/ tissue samplesvs normal tissue and cell lines Overexpression of miR-124suppressed Rac1 protein expression 8 Rac1 TaqMan RT-PCR, Cellproliferation and invasion assay,Dual Luciferase assay, Rac1rescue assay and western blot. Geng et al.(2014)