miR-199a-3p, miR-127-3p | OS cell line vs osteoblast cells | Inhibits cell growth, migration, regulate cell cycle | 19, 14 respectively | mTOR, stat3 | Microarray, qRT-PCR | Duan et al. (2011) |
miR-34 family (34a, 34b, and 34c) | OS cell line vs patients samples | P53 dependent manner cell cycle arrest and apoptosis | 1, 11 respectively | CDK6, E2F3, CyclinE2, Bcl-2 | qRT-PCR, 3UTR luciferase reporter assay, FACS analysis, DNA methylation assay | He et al. (2009); Wu et al.(2013) |
miR-382, miR- 134, miR-369- 3p and miR-544 | OS vs normal bone, OS cell line vs osteoblast | 14q32 miRNAs decreased cMYC levels and induced apoptosis | 14 | cMYC | Microarray, qRT-PCR, Western blot | Thayanithy et al. (2012) |
miR-143 | OS patients tissue sample,MG63 and U2OS cell lines vs normal osteoblast and HEK293 cell line | miR-143 may exert its proapoptoticfunction via inhibiting Bcl-2 expression. | 5 | Bcl2 | 3 UTR luciferase reporter assay, qRT-PCR, Western Blot and Tumorigenicity assay in nude mice | Zhang et al. (2010) |
miR-145 | Human OS tissues vs normal boneTissues, MG63 vs ECV304 cell lines | miR-145 down regulate VEGF attranslational level by partially binding to VEGF 3 UTR. | 4 | BCL2L2, MCL-1,IGF1R and ME, p-Akt and FAK | qRT-PCR, FACS analysis, Cell Proliferation Assay, Boydenchamber assay, Western blotting and Hierarchical cluster analysis | Zhao et al. (2013) |
miR-24 | Human OS vs non-cancerousadjacent tissues, MG63, 143Band hFOB1.19 | Overexpression of miR-24 downregulatedLPAATb expression in OScells. | 9 | LPAATb | Immunofluorescence andImmunohist. staining,Computational analysis byprogram:TargetScan5.1, qRTPCRanalysis, Fluorescentreporter assay, Cell viabilityassay, FACS | Song et al.(2013) |
miR-16 | HOS, KHOS, U2OS, and MG-63 vs HOB cell line andEighteen OS patient samplesvs normal tissues | Overexpression of miR-16suppresses OS cell proliferation andtumor growth by inhibition of Raf1 MEK1/2 ERK1/2 pathway. | 13 | IGF1R | Cell proliferation assay and cellcycle analysis, Western blotting,Tumor growth assay, luciferasereporter assay, | Chen et al.(2013) |
miR-199b-5p | Eight FFPE Patients vstissue samples, ES97, HS3.T,SJSA-1, andMG63 vs normal cell line | Inhibition of Notch and HES1signaling by miR-199b-5p as apotential therapeutic strategy toprevent metastasis in human OS. | 9 | HES1 andNotchsignaling | Microarray analysis and qRTPCR | Won et al.(2013) |
miR-124 | MG-63, U2OS, SOSP-9607,and SAOS-2/ tissue samplesvs normal tissue and cell lines | Overexpression of miR-124suppressed Rac1 protein expression | 8 | Rac1 | TaqMan RT-PCR, Cellproliferation and invasion assay,Dual Luciferase assay, Rac1rescue assay and western blot. | Geng et al.(2014) |