Table 2. Changes in spermatocrit, sperm density, and milt production in the mature grass puffer, Takifugu niphobles, after an injection of carp pituitary extract (CPE)*
Exp. group (mg/kg BW) | Hours after injection | Spermatocrit (%) | Sperm density (×105 spz/μL milt) | Milt production (mL/kg BW) |
Cont. | 0 | 75.0 ± 0.79Aa | 8.0 ± 0.84Aa | 57.8 ± 17.19Aa |
5 | 75.2 ± 0.84Aa | 7.9 ± 0.93Aa | 55.7 ± 13.75Ab |
10 | 75.0 ± 0.71Aa | 8.3 ± 1.31Aa | 58.1 ± 11.32Ab |
20 | 74.8 ± 0.84Aa | 7.8 ± 0.98Aa | 56.9 ± 12.85Ab |
Cont. | 24 | 72.0 ± 9.85Aa | 6.5 ± 0.30Ab | 57.6 ± 12.94Ca |
5 | 60.7 ± 26.5Bb | 4.8 ± 0.71Bb | 79.0 ± 42.40Ba |
10 | 63.7 ± 7.02Bb | 4.7 ± 0.93Bb | 83.8 ± 27.25Ba |
20 | 64.7 ± 9.24Bb | 4.1 ± 1.12Cb | 89.4 ± 72.18Aa |
Cont. | 48 | 64.7 ± 11.93Ab | 6.7 ± 0.67Ab | 17.1 ± 9.53Bb |
5 | 55.3 ± 21.96Bc | 4.8 ± 2.63Bb | 32.8 ± 30.34Ac |
10 | 61.3 ± 11.73Ab | 4.6 ± 2.15Bb | 37.9 ± 45.93Ac |
20 | 51.3 ± 19.35Bc | 4.1 ± 2.51Cb | 13.2 ± 9.34Bc |
Means ± SD (n = 50) of triplicate groups. Data were analyzed using one- and two-way ANOVA.
Different capital letters on the values indicate statistically significant differences among doses at each time after injection (P < 0.05).
Different small letters on the values indicate statistically significant differences among times after injection for each dose (P < 0.05). Cont.: control group; Exp.: experimental group; spz.: spermatozoa.