A1 | Normal swimming, opercular movement and normal general movement |
A2 | Swimming speed slowed, rolling from side to side |
A3 | Partial loss of equilibrium, swimming erratic |
A4 | Complete loss of equilibrium, swimming perfectly inside out, pectoral fin, pelvic fin and dorsal fin movement stop |
A5 | Little sedation, anal fin and tail fin movement stop |
A6 | Perfect sedation, only opercular movement, when flip the sample, no reaction observed |
A7 | Opercular movement ceased |
Recovery |
Stage | Characteristic behavior |
R1 | Resume opercular movement |
R2 | Preferential movement of pectoral fin and tail fin |
R3 | Dorsal fin, pelvic fin and anal fin movement |
R4 | Swimming perfectly inside out |
R5 | Swimming erratic, recovery of balance, When flip the sample, observed to flip themselves |
R6 | Normal swimming, responsiveness to visual stimuli |