Table 1 Stages of anesthesia induction and recovery in clove oil efficacy tests performed in olive flounder, Paralichthys olivaceus

Anesthesia
Stage Characteristic behavior
A1 Normal swimming, opercular movement and normal general movement
A2 Swimming speed slowed, rolling from side to side
A3 Partial loss of equilibrium, swimming erratic
A4 Complete loss of equilibrium, swimming perfectly inside out, pectoral fin, pelvic fin and dorsal fin movement stop
A5 Little sedation, anal fin and tail fin movement stop
A6 Perfect sedation, only opercular movement, when flip the sample, no reaction observed
A7 Opercular movement ceased
Recovery
Stage Characteristic behavior
R1 Resume opercular movement
R2 Preferential movement of pectoral fin and tail fin
R3 Dorsal fin, pelvic fin and anal fin movement
R4 Swimming perfectly inside out
R5 Swimming erratic, recovery of balance, When flip the sample, observed to flip themselves
R6 Normal swimming, responsiveness to visual stimuli