Table 5 Inability rates of far eastern catfish, Silurus asotus at 720 days after storage in two diluents and different concentrations of two cryoprotectants*
Diluent | Cryoprotectant | Concentrations(%) | | Inability rate of spermatozoa (%) |
| Type A | Type B |
Fresh sperm | - | - | 5.4±0.42a | 9.0±0.42a |
no treatment | No treatment | 0 | 5.6±0.58a | 91.2±8.96a |
Alsever'ssolution | No treatment | 0 | 5.1±0.66a | 87.6±7.19a |
Dimethylsulfoxide(DMSO) | 5 | 5.3±0.61a | 63.8±0.37a |
10 | 5.6±0.78a | 61.8±0.38b |
15 | 5.7±0.91a | 61.4±0.43b |
20 | 5.1±0.64a | 61.5±0.40b |
Ethyleneglycol | 5 | 5.4±0.49a | 49.4±0.55a |
10 | 5.5±0.71a | 47.5±0.64b |
15 | 5.3±0.75a | 47.0±0.71b |
20 | 5.7±0.66a | 47.2±0.38b |
0.3Mglucose | No treatment | 0 | 5.8±0.59a | 87.1±9.88a |
Dimethylsulfoxide(DMSO) | 5 | 5.1±0.94a | 75.4±0.79a |
10 | 5.9±0.86a | 73.1±0.91b |
15 | 5.2±0.82a | 73.4±0.88b |
20 | 5.5±0.47a | 73.6±0.98b |
Ethyleneglycol | 5 | 5.7±0.55a | 52.4±0.98a |
10 | 5.2±0.76a | 50.9±1.01b |
15 | 5.9±0.82a | 50.6±0.99b |
20 | 5.1±0.92a | 50.1±1.05b |
* The temperature of storage is –80℃. Mean±SD (n=100) values are shown. Means sharing the same letter superscript on given dates are not significantly different (P>0.05). Experiments are triplicate. For detail external morphology, refer to Fig. 2-C (type A) and 2-D (type B).