Table 2 Specific conditions of PCRs used in this study

Gene Temperature & time denature / annealing / extension Number of cycle
Cgα 94℃, 30sec / 60℃, 40sec / 72℃, 40sec 27
LH-β 94℃, 30sec / 68℃, 40sec / 72℃, 40sec 28
FSH-β 94℃, 30sec / 68℃, 40sec / 72℃, 40sec 29
KiSS-1 94℃, 30sec / 68℃, 40sec / 72℃, 40sec 35
GnRH 94℃, 30sec / 64℃, 40sec / 72℃, 40sec 35
GAPDH 94℃, 30sec / 62℃, 40sec / 72℃, 40sec 28