Gene | Temperature & time | Number of cycle |
---|---|---|
Cgα | 94℃, 30sec / 60℃, 40sec / 72℃, 40sec | 27 |
LH-β | 94℃, 30sec / 68℃, 40sec / 72℃, 40sec | 28 |
FSH-β | 94℃, 30sec / 68℃, 40sec / 72℃, 40sec | 29 |
KiSS-1 | 94℃, 30sec / 68℃, 40sec / 72℃, 40sec | 35 |
GnRH | 94℃, 30sec / 64℃, 40sec / 72℃, 40sec | 35 |
GAPDH | 94℃, 30sec / 62℃, 40sec / 72℃, 40sec | 28 |