Table 1 Effects of siRNA #693-mediated knockdown of BiP/GRP78 on induction of ER stress and meiotic maturation of pig oocytes during IVM

No. of oocytesexamined % of oocytes (n)
GV GVBD M I M II
Con 145 3.9±4.5 (3) 2.3±1.8 (4) 16.1±5.4 (26) a 77.8±5.3 (112) a
N.C siRNA 143 1.5±2.0 (2) 4.3±5.5 (6) 28.1±7.5 (41) b 66.1±2.8 (94) b
siRNA #693 162 3.9±2.6 (5) 9.6±9.2 (11) 54.0±11.7 (90) d 32.5±10.1 (56) d
siRNA #693+TUDCA 126 3.8±3.1 (6) 5.1±6.3 (9) 17.2±6.9 (27) a 73.9±3.1 (84) a
siRNA #693+Mela 155 1.4±2.9 (2) 2.6±3.5 (5) 18.8±5.4 (29) a 77.2±2.9 (119) a
The table represents the % of oocytes in different stages of oocyte maturation in control and siRNA #693-transfected COCs with or without TUDCA (200 μM) and melatonin (0.1 μM) treatment. Data are represented as mean±SD of three independent experiments. a~d superscripts denote a significant difference among groups (p<0.05).