Table 1 Effects of siRNA #693-mediated knockdown of BiP/GRP78 on induction of ER stress and meiotic maturation of pig oocytes during IVM
| No. of oocytesexamined | % of oocytes (n) |
GV | GVBD | M I | M II |
Con | 145 | 3.9±4.5 (3) | 2.3±1.8 (4) | 16.1±5.4 (26) a | 77.8±5.3 (112) a |
N.C siRNA | 143 | 1.5±2.0 (2) | 4.3±5.5 (6) | 28.1±7.5 (41) b | 66.1±2.8 (94) b |
siRNA #693 | 162 | 3.9±2.6 (5) | 9.6±9.2 (11) | 54.0±11.7 (90) d | 32.5±10.1 (56) d |
siRNA #693+TUDCA | 126 | 3.8±3.1 (6) | 5.1±6.3 (9) | 17.2±6.9 (27) a | 73.9±3.1 (84) a |
siRNA #693+Mela | 155 | 1.4±2.9 (2) | 2.6±3.5 (5) | 18.8±5.4 (29) a | 77.2±2.9 (119) a |
The table represents the % of oocytes in different stages of oocyte maturation in control and siRNA #693-transfected COCs with or without TUDCA (200 μM) and melatonin (0.1 μM) treatment. Data are represented as mean±SD of three independent experiments. a~d superscripts denote a significant difference among groups (p<0.05).