Table 1 Characteristics of unfertilized and fertilized eggs of grass puffer, Takifugu niphobles at different water temperatures*

Characteristics of egg	Color			Type
Characteristics of egg	Light yellowish			Unadhesive and demersal
Egg weight (mg)	0.09±0.002^a
Egg size (mm)
		Water temperature for egg development (℃)
		18	20	22	24
Unfertilized egg	Major axis	1.05±0.041^a
	Minor axis	1.01±0.038^a
	Extracellular matrix	0.09±0.005^a
Fertilized egg	Major axis	1.39±0.032^a	1.43±0.039^b	1.45±0.064^b	1.46±0.043^b
	Minor axis	1.36±0.035^a	1.41±0.048^b	1.42±0.053^b	1.43±0.052^b
	Extracellular matrix	0.09±0.009^a	0.09±0.005^a	0.11±0.009^a	0.09±0.008^a

*Each value is presented as mean±SE of triplicated groups (n=30). Means in rows with the different superscript letter are significantly different (p<0.05). Fertilized egg sizes were measured for blastodisc stage at each water temperature.