Table 1 Information for oligonucleotide primers utilized for quantitative real-time PCR analysis

Gene	Primer sequence(5’ → 3’)	Tm(°C)	PCR product size (bps)
Gjb2 (Cx26)(NM_001004099)	(F) TCCTCTTCATCTTCCGCATC(R) CCGTTTCTTTTCGTGTCTCC	55	233
Gjb3 (Cx31)(NM_019240)	(F) TTGAGCGGTGTGAACCAGTA(R) TGTTGGAGATGGGGAAGAAG	58	193
Gjb5 (Cx31.1)(NM_019241)	(F) CATCGTCTGCATCCTGCTTA(R) ATGAGGTCGCTTGAGAGGAA	55	165
Gjb1 (Cx32)(NM_017251)	(F) AGAATCATGGTGCTGGTGGT(R) CCTCAAGCCGTAGCATTTTC	58	235
Gja6 (Cx33)(NM_019308)	(F) TGAGAGGCAGATTGCTGCTA(R) AGACACCATTGACACCACCA	58	221
Gjd2 (Cx36)(NM_019281)	(F) TCTGGAGATTGGGTTTCTGG(R) CGGACAGCCAGTTTGATCTT	58	231
Gja4 (Cx37)(NM_021654)	(F) AGTGTCTGTACCTTGGATGCC(R) CAGCACACTTAGCCAAGAGC	55	223
Gja5 (Cx40)(NM_019280)	(F) ATACCATTCAGCCTGGTTGC(R) CGGCCTCTTTAGCTTTCTCA	58	189
Gja1 (Cx43)(NM_012567)	(F) AGCAAGCTAGCGAGCAAAAC(R) GAGTTCATGTCCAGCAGCAA	55	151
Gja7 (Cx45)(NM_001085381)	(F) GATCATCCTGGTTGCTACTC(R) GATCCTCTTCATGGTCCTCT	58	173
Gja8 (Cx50)(NM_153465.2)	(F) CCACTCCATTGCAGTTTCCT(R) AGAAGGCAGGGTTTCTTGGT	58	211
Actb(NM_031144.3)	(F) ACCTTCAACACCCCAGCCATGTACG(R) CTGATCCACATCTGCTGGAAGGTGG	55	698

PCR, polymerase chain reaction, Cx, connexin; Gja, gap junction protein, alpha; Gjb, gap junction protein, beta; Gjd, gap junction protein, delta; Actb, actin, beta.