Table 2 Eggs and embryonic development of R. ocellatus at water temperature 21.5±1.0°C

Stage	Elapsed time	Characters	Fig. 3
Zygote period
Fertilization	0 hr 00 min	The egg absorbs water	A
Cell cleave period
Blastodisc	0 hr 35 min	Blastodisc is formed	B
Two celled egg	1 hr 00 min	2-1 array of blastomeres	C
Four celled egg	2 hr 00 min	2-2 array of blastomeres	D
Eight celled egg	2 hr 30 min	2-4 array of blastomeres	E
Sixteen celled egg	4 hr 30 min	4-4 array of blastomeres	F
Thirty-two celled egg	6 hr 30 min	4-8 array of blastomeres	G
Sixty-four celled egg	9 hr 30 min	8-8 array of blastomeres	H
Blastula period
Morula	11 hr 30 min	The size of the blastomere is getting smaller	I
Blastula	13 hr 00 min	The surface of the blastomere coincides with the egg yolk	J
Gastrula period
Gastrulation 1/3	17 hr 30 min	Covered 1/3 of egg yolk	K
Gastrulation 2/3	23 hr 30 min	Covered 2/3 of egg yolk	L
Embryonic period	26 hr 00 min	Development of embryo	M
Embryo just before hatching	40 hr 00 min	The tail is getting longer	N
Hatching period	50 hr 00 min	There was no movement of the hatched larvae.	O