Table 1 All primer sequences used for RT-PCR and RT-qPCR

Genes	Primer sequence (5’-3’)	Length (bp)	GenBank Acc No.	Annealing temp (℃)
XBP1	F-GGCAGAGACCAAGGGGAATGR-GGGTCGACTTCTGGGAGCTG	263	FJ213449.1	60
CHOP	F-AAGACCCAGGAAACGGAAACR-TCCAGGAAAGGTCAGCAGTA	261	NM_001144845.1	58
BiP	F-ACCAATGACCAAAATCGCCTR-GTGACTTTCCAGCCACTCAA	246	J03214.1	58
ATF4	F-TGAGCCCTGACTCCTATCTGR-TCCAGCTCTTTACATTCGCC	277	NM_001123078.1	58
GRP94	F-CTGCTGAAGGGGAAGTTACCR-ATCATCTGAGTCCACAACGC	197	Y09136.1	58
Bcl2L1	F-GTTGACTTTCTCTCCTACAAGCR-GGTACCTCAGTTCAAACTCATC	277	NM_214285.1	55
Bax	F-ACTGGACAGTAACATGGAGCR-GTCCCAAAGTAGGAGAGGAG	294	XM003127290.3	55
Caspases-3	F-GAGGCAGACTTCTTGTATGCR-CATGGACACAATACATGGAA	237	NM_214131	60
GAPDH	F-GGGCATGAACCATGAGAAGTR-AAGCAGGGATGATGTTCTGG	230	AF017079	58

Xbp1, x-box binding protein 1 ; CHOP, homologous protein ; BiP, binding protein; ATF4, activating transcription factor 4; GRP94, glucose-regulated protein 94 ; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.