Table 1. Primers for RT-qPCR of ctsb, ctsd, ctsl, lpl and β-actin for Japanese eel

Gene		Primer sequence	Product size
ctsb	Forward	5′-AAGAACCCATTGTCACCCC-3′	136 bp
	Reverse	5′-GTGTCTATCAGCACGTCACTG-3′
ctsd	Forward	5′-CAGGGAGAGTACATGGTTGAC-3′	115 bp
	Reverse	5′-GGGACTCCTTGAGAATGTACTG-3′
ctsl	Forward	5′-GTGACCATGACGCTGTATTTG-3′	104 bp
	Reverse	5′-TTCCATGAGTCCCAATGCTC-3′
lpl	Forward	5′-GATGTTTGCTATATTGTCGCTGG-3′	115 bp
	Reverse	5′-TCTCAAACATTCCACTCACCG-3′
β -actin	Forward	5′-AGTATTTGCGCTCGGGTG-3′	225 bp
	Reverse	5′-CAGCCTTCCTTCCTGGGT-3′