Table 1. Primer information for real-time PCR analysis
Gene
Sequence
(5’ → 3’)
T
m
(°C)
PCR product
(bps)
Star
(NM_011485.5)
(F) GCTGGAAGTCCCTCCAAGACT
(R) GCCACCCCTTCAGGTCAATA
60
61
Cyp11a1
(AF195119.1)
(F) CGATACTCTTCTCATGCGAG
(R) CTTTCTTCCAGGCATCTGAAC
52
126
Cyp17a1
(NM_007809.3)
(F) GGATCCGATACTGACTACCA
(R) GGCTGGAGAGAGATTCACTA
50
212
Hsd3b1
(NM_008293.4)
(F) GGATCATCAAGATGTTGGTGC
(R) TGGTTTCTGGTCGGAATACTT
55
78
Hsd17b3
(NM_008291.3)
(F) ATGGCATCGGGAAAGCCTAT
(R) CTCTTCTGCAATGGTCTGTAGC
60
111
Gapdh
(NM_008084.3)
(F) GGCCATGAGGTCCACCACCCTGTTGCTGTA
(R) CCCCTGGCCAAGGTCATCCATGACAACTTT
60
513