Table 1 Water quality items for this experiment in river puffer, Takifugu obscurus, tiger puffer, T. rubripes, hybrids (river puffer ♀×tiger puffer ♂), and hybrid triploids*

Test parameters	Condition
	0 psu	15 psu	30 psu
Temperature (°C)	26±0.5	26±0.5	26±0.5
pH	7.1±0.65	7.1±0.78	7.1±1.02
DO (dissolved oxygen: mg/L; saturated concentration in 25°C)	7.6	7.5	7.5
Ammonia (ppm)	0.01	0.06	0.06
Nitric acid (ppm)	1.8±0.14	1.8±0.12	1.7±0.19
Nitrous acid (ppm)	0.01	0.01	0.02