Table 2. Bioactivity of eel LH receptors in cells expressing activating receptor mutants

Eel LH receptors cAMP responses
Basal1) (nM/104 cells) EC50 (ng/mL) Rmax2) (nM/104 cells)
Eel LHR-wt 1.2±0.5 (1-fold) 18.9 (15.3 to 24.9)3) 87.5±2.2(1-fold)
Eel LHR-M410T 4.8±0.3 (4.0-fold) 8.7 (5.5 to 20.1) 57.3±2.6 (0.65-fold)
Eel LHR-L469R 22.9±1.5 (19.1-fold) 3.8 (2.4 to 8.1) 46.2±1.1 (0.52-fold)
Eel LHR-D590Y 9.3±0.8 (7.8-fold) 77.3 (52.5 to 147) 85.9±4.1 (0.98-fold)
Basal average level of cAMP without treatment with agonist.
Rmax average level of cAMP/104 cells.
Geometric mean (95% confidence limit).