Table 2. Bioactivity of eel LH receptors in cells expressing activating receptor mutants
Eel LH receptors | cAMP responses |
Basal1) (nM/104 cells) | EC50 (ng/mL) | Rmax2) (nM/104 cells) |
Eel LHR-wt | 1.2±0.5 (1-fold) | 18.9 (15.3 to 24.9)3) | 87.5±2.2(1-fold) |
Eel LHR-M410T | 4.8±0.3 (4.0-fold) | 8.7 (5.5 to 20.1) | 57.3±2.6 (0.65-fold) |
Eel LHR-L469R | 22.9±1.5 (19.1-fold) | 3.8 (2.4 to 8.1) | 46.2±1.1 (0.52-fold) |
Eel LHR-D590Y | 9.3±0.8 (7.8-fold) | 77.3 (52.5 to 147) | 85.9±4.1 (0.98-fold) |
Basal average level of cAMP without treatment with agonist.
Rmax average level of cAMP/104 cells.
Geometric mean (95% confidence limit).