Table 3. Bioactivity of eel LH receptors in cells expressing inactivating receptor mutants

Eel LH receptors cAMP responses
Basal1) (nM/104 cells) EC50 (ng/ml) Rmax2) (nM/104 cells)
Eel LHR-wt 2.6±0.1 31.7 (26.1 to 40.6)3) 69.5±1.5(1-fold)
Eel LHR-D417N 1.2±0.1 11.9 (9.4 to 16.5) 22.7±0.6(0.33-fold)
Eel LHR-Y558F 0.7±0.1 13.8 (12.2 to 15.7) 17.3±0.2 (0.25-fold)
Basal average level of cAMP without treatment with agonist.
Rmax average level of cAMP/104 cells.
Geometric mean (95% confidence limit).