Table 2. Primers and product size for PCR genotyping and T7E1 assay
Target of sgRNA
PCR size
T7E1 assay
ID primer
Sequence
ints14
exon2
436 bp
336±@ bp
100±@ bp
Ex2 Fp
CATAACTGTTCTTCAGCGTGTAAA
Ex3 Rp
CACTCCTGCTGTACTACATTACT