Table 1. Accession numbers, primer sequences and product sizes used in this study

Gene	Accession number	Sequence of the primers	Product size (bp)
tLHβ	XM_063278267.1	F 5’- GGA GCT CAC TGA CCA CCA TCR 5’- GTA CAC TGT AGA TGC CTG GCA C	428
pLHβ	NM_012858.2	F 5’- GGT ATC AAG AAT GGA GAG GCR 5’- GCA TCA GAA GAG GAG AAG GC	685
Cgα	NM_053918.2	F 5’- ATA CTT CTC CAA GCT GGG TGR 5’- CGA CAC TCA GTG CCA TCG CA	294
LHCGR	NM_012978.2	F 5’- GCC AGA CTA TCT CTC ACC TAT CR 5’- AGT GTG ACA GAC TCG TTA TTC	383
PACAP	NM_016989.2	F 5’- GCA AGA CTT CTA CGA CTG GR 5’- CTG GCA ACT CAT CGC TAC	396
GnRH	NM_012767.2	F 5’- ACT GAT GGC CGC TGT TGT TCR 5’- GCC CAG CTT CCT CTT CAA TC	248
Kisspeptin	NM_181692.2	F 5’- AAT GGC ACC TGT GGT GAA CCR 5’- GGA GTT CCA GTT GTA GGC TG	271
GAPDH	NM_017008.4	F 5’- CCA TCA CCA TCT TCC AGG AGR 5’- CCT GCT TCA CCC ACC TTC TTG	576