Table 2. PCR conditions used in this study

Gene	Temperature & time denature / annealing / extension	Number of cycles
tLHβ	95°C, 60 sec / 54°C, 60 sec / 72°C, 60 sec	35
pLHβ	95°C, 60 sec / 51°C, 60 sec / 72°C, 60 sec	35
Cgα	95°C, 60 sec / 53°C, 60 sec / 72°C, 60 sec	38
LHCGR	95°C, 35 sec / 50°C, 35 sec / 72°C, 45 sec	35
PACAP	95°C, 35 sec / 52°C, 35 sec / 72°C, 45 sec	32
GnRH	95°C, 35 sec / 58°C, 35 sec / 72°C, 45 sec	35
Kisspeptin	95°C, 35 sec / 60°C, 35 sec / 72°C, 45 sec	41
GAPDH	95°C, 60 sec / 55°C, 35 sec / 72°C, 60 sec	28